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elisa micro-titer plate reader  (Agilent technologies)


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    Structured Review

    Agilent technologies elisa micro-titer plate reader
    Elisa Micro Titer Plate Reader, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/micro-elisa+plate+reader/10__21608_slash_ejmm__2024__290825__1250-57-10-14
    Average 90 stars, based on 1 article reviews
    elisa micro-titer plate reader - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Incubation:

    Article Title: Effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE47) on the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism, and differences in the action of parent BDE-47 and its hydroxylated metabolites, 5-OH-BDE-47 and 6-OH-BDE47, on steroid secretion by luteal cells.
    Article Snippet: 13 In this study we determined the effects of BDE-47 on the expression and activity of phase I 14 (CYP2B1/2) and phase II (SULT1A and COMT) enzymes, and assessed the actions of BDE15 47 and its metabolites on luteal steroidogenesis.. Luteal cells collected during early (ELP), 16 middle (MLP) and late (LLP) luteal phase were exposed to BDE-47 (0.5, 25, and 50 ng/ml) or 17 metabolites (2.5, 5 and 25 ng/ml).. BDE-47 decreased CYP2B1/2 activity and expression but 18 had no effect on SULT1A or COMT.

    Article Title: Hexachlorobenzene and pentachlorobenzene accumulation, metabolism and effect on steroid secretion and on CYP11A1 and CYP19 expression in cultured human placental tissue.
    Article Snippet: Hexachlorobenzene and pentachlorobenzene accumulation and the effect on CYP1A1, SULT1A, COMT and steroid secretion in term placental tissue were determined.. Explants of placental tissue were exposed to between 0.02 and 2 ng/ml HCBz or PeCBz for 6–72 h. Accumulation was measured by capillary gas chromatography and quadrupole mass spectrometry.. CYP1A1, SULT1A, COMT activity and progesterone secretion were analysed by EIA.

    Article Title: Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary.
    Article Snippet: The aim of the current study was to determine metabolism of polybrominated diphenyl ether (BDE-47) in the porcine ovary.. We analyzed the activity and expression of enzymes involved in phase I (CYP1A1 and CYP2B1/2) and phase II (SULT1A and COMT) of BDE-47 metabolism.. Basal CYP1A1 and CYP2B1/2 activity increased during culture.

    Article Title: Nitrophenols disrupt the expression and activity of biotransformation enzymes (CYP3A and COMT) in chicken ovarian follicles in vivo and in vitro.
    Article Snippet: The reaction was terminated by the addition of 1 M NaOH, and absorbance was measured at a wavelength of 520 nm using a micro-ELISA plate reader (BioTek Instruments).

    Article Title: A mixture of persistent organic pollutants detected in human follicular fluid increases progesterone secretion and mitochondrial activity in human granulosa HGrC1 cells.
    Article Snippet: Absorbance was measured at 490 nm using a micro-ELISA plate reader (BioTek Instruments).

    Article Title: Nitrophenols disrupt the expression and activity of biotransformation enzymes (CYP3A and COMT) in chicken ovarian follicles in vivo and in vitro.
    Article Snippet: The absorbance was measured after a 30-min incubation period at a wavelength of 520 nm using a micro-ELISA plate reader (BioTek Instruments).

    Negative Control:

    Article Title: Effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE47) on the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism, and differences in the action of parent BDE-47 and its hydroxylated metabolites, 5-OH-BDE-47 and 6-OH-BDE47, on steroid secretion by luteal cells.
    Article Snippet: 13 In this study we determined the effects of BDE-47 on the expression and activity of phase I 14 (CYP2B1/2) and phase II (SULT1A and COMT) enzymes, and assessed the actions of BDE15 47 and its metabolites on luteal steroidogenesis.. Luteal cells collected during early (ELP), 16 middle (MLP) and late (LLP) luteal phase were exposed to BDE-47 (0.5, 25, and 50 ng/ml) or 17 metabolites (2.5, 5 and 25 ng/ml).. BDE-47 decreased CYP2B1/2 activity and expression but 18 had no effect on SULT1A or COMT.

    Article Title: Hexachlorobenzene and pentachlorobenzene accumulation, metabolism and effect on steroid secretion and on CYP11A1 and CYP19 expression in cultured human placental tissue.
    Article Snippet: Hexachlorobenzene and pentachlorobenzene accumulation and the effect on CYP1A1, SULT1A, COMT and steroid secretion in term placental tissue were determined.. Explants of placental tissue were exposed to between 0.02 and 2 ng/ml HCBz or PeCBz for 6–72 h. Accumulation was measured by capillary gas chromatography and quadrupole mass spectrometry.. CYP1A1, SULT1A, COMT activity and progesterone secretion were analysed by EIA.

    Article Title: Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary.
    Article Snippet: The aim of the current study was to determine metabolism of polybrominated diphenyl ether (BDE-47) in the porcine ovary.. We analyzed the activity and expression of enzymes involved in phase I (CYP1A1 and CYP2B1/2) and phase II (SULT1A and COMT) of BDE-47 metabolism.. Basal CYP1A1 and CYP2B1/2 activity increased during culture.

    Article Title: Nitrophenols disrupt the expression and activity of biotransformation enzymes (CYP3A and COMT) in chicken ovarian follicles in vivo and in vitro.
    Article Snippet: The reaction was terminated by the addition of 1 M NaOH, and absorbance was measured at a wavelength of 520 nm using a micro-ELISA plate reader (BioTek Instruments).

    Article Title: A mixture of persistent organic pollutants detected in human follicular fluid increases progesterone secretion and mitochondrial activity in human granulosa HGrC1 cells.
    Article Snippet: Absorbance was measured at 490 nm using a micro-ELISA plate reader (BioTek Instruments).

    Article Title: Nitrophenols disrupt the expression and activity of biotransformation enzymes (CYP3A and COMT) in chicken ovarian follicles in vivo and in vitro.
    Article Snippet: The absorbance was measured after a 30-min incubation period at a wavelength of 520 nm using a micro-ELISA plate reader (BioTek Instruments).



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    Image Search Results


    Gender-wise distribution of CHIKV cases based on ELISA and RT-PCR.

    Journal: PLOS ONE

    Article Title: Molecular identification and phylogenetic analysis of chikungunya virus among dengue-negative patients in Kolkata, India

    doi: 10.1371/journal.pone.0301644

    Figure Lengend Snippet: Gender-wise distribution of CHIKV cases based on ELISA and RT-PCR.

    Article Snippet: All the assay was performed and optical density (OD) was measured at 450nm using the kit specific protocol provided by the manufacturer with the help of semi-automatic ELISA micro-plate reader and washer made by Thermo Fisher Scientific (India).

    Techniques: Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction

    Month-wise distribution of CHIKV cases based on ELISA (2A) and RT-PCR (2B).

    Journal: PLOS ONE

    Article Title: Molecular identification and phylogenetic analysis of chikungunya virus among dengue-negative patients in Kolkata, India

    doi: 10.1371/journal.pone.0301644

    Figure Lengend Snippet: Month-wise distribution of CHIKV cases based on ELISA (2A) and RT-PCR (2B).

    Article Snippet: All the assay was performed and optical density (OD) was measured at 450nm using the kit specific protocol provided by the manufacturer with the help of semi-automatic ELISA micro-plate reader and washer made by Thermo Fisher Scientific (India).

    Techniques: Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction

    District-wise distribution of CHIKV cases based on ELISA (3A) and RT-PCR(3B). In this figure, we have analysed the district-wise prevalence rate of CHIKV by considering the total number of CHIKV sample tested and the samples showed positive results in both ELISA and RT-PCR assay. The samples showed ELISA positive results for CHIKV were not considered further for RT-PCR assay.

    Journal: PLOS ONE

    Article Title: Molecular identification and phylogenetic analysis of chikungunya virus among dengue-negative patients in Kolkata, India

    doi: 10.1371/journal.pone.0301644

    Figure Lengend Snippet: District-wise distribution of CHIKV cases based on ELISA (3A) and RT-PCR(3B). In this figure, we have analysed the district-wise prevalence rate of CHIKV by considering the total number of CHIKV sample tested and the samples showed positive results in both ELISA and RT-PCR assay. The samples showed ELISA positive results for CHIKV were not considered further for RT-PCR assay.

    Article Snippet: All the assay was performed and optical density (OD) was measured at 450nm using the kit specific protocol provided by the manufacturer with the help of semi-automatic ELISA micro-plate reader and washer made by Thermo Fisher Scientific (India).

    Techniques: Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction

    Mean weight, blood sugar level and age of patients based on the RT-PCR and serological detection of CHIKV.

    Journal: PLOS ONE

    Article Title: Molecular identification and phylogenetic analysis of chikungunya virus among dengue-negative patients in Kolkata, India

    doi: 10.1371/journal.pone.0301644

    Figure Lengend Snippet: Mean weight, blood sugar level and age of patients based on the RT-PCR and serological detection of CHIKV.

    Article Snippet: All the assay was performed and optical density (OD) was measured at 450nm using the kit specific protocol provided by the manufacturer with the help of semi-automatic ELISA micro-plate reader and washer made by Thermo Fisher Scientific (India).

    Techniques: Enzyme-linked Immunosorbent Assay