elisa micro-titer plate reader (Agilent technologies)
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Elisa Micro Titer Plate Reader, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/micro-elisa+plate+reader/10__21608_slash_ejmm__2024__290825__1250-57-10-14
Average 90 stars, based on 1 article reviews
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Incubation:Article Title: Effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE47) on the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism, and differences in the action of parent BDE-47 and its hydroxylated metabolites, 5-OH-BDE-47 and 6-OH-BDE47, on steroid secretion by luteal cells. Article Snippet: 13 In this study we determined the effects of BDE-47 on the expression and activity of phase I 14 (CYP2B1/2) and phase II (SULT1A and COMT) enzymes, and assessed the actions of BDE15 47 and its metabolites on luteal steroidogenesis.. Luteal cells collected during early (ELP), 16 middle (MLP) and late (LLP) luteal phase were exposed to BDE-47 (0.5, 25, and 50 ng/ml) or 17 metabolites (2.5, 5 and 25 ng/ml).. BDE-47 decreased CYP2B1/2 activity and expression but 18 had no effect on SULT1A or COMT. Article Title: Hexachlorobenzene and pentachlorobenzene accumulation, metabolism and effect on steroid secretion and on CYP11A1 and CYP19 expression in cultured human placental tissue. Article Snippet: Hexachlorobenzene and pentachlorobenzene accumulation and the effect on CYP1A1, SULT1A, COMT and steroid secretion in term placental tissue were determined.. Explants of placental tissue were exposed to between 0.02 and 2 ng/ml HCBz or PeCBz for 6–72 h. Accumulation was measured by capillary gas chromatography and quadrupole mass spectrometry.. CYP1A1, SULT1A, COMT activity and progesterone secretion were analysed by EIA. Article Title: Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary. Article Snippet: The aim of the current study was to determine metabolism of polybrominated diphenyl ether (BDE-47) in the porcine ovary.. We analyzed the activity and expression of enzymes involved in phase I (CYP1A1 and CYP2B1/2) and phase II (SULT1A and COMT) of BDE-47 metabolism.. Basal CYP1A1 and CYP2B1/2 activity increased during culture. Article Title: Nitrophenols disrupt the expression and activity of biotransformation enzymes (CYP3A and COMT) in chicken ovarian follicles in vivo and in vitro. Article Snippet: The reaction was terminated by the addition of 1 M NaOH, and absorbance was measured at a wavelength of 520 nm using a Article Title: A mixture of persistent organic pollutants detected in human follicular fluid increases progesterone secretion and mitochondrial activity in human granulosa HGrC1 cells. Article Snippet: Absorbance was measured at 490 nm using a Article Title: Nitrophenols disrupt the expression and activity of biotransformation enzymes (CYP3A and COMT) in chicken ovarian follicles in vivo and in vitro. Article Snippet: The absorbance was measured after a 30-min incubation period at a wavelength of 520 nm using a Negative Control:Article Title: Effects of 2,2',4,4'-tetrabromodiphenyl ether (BDE47) on the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism, and differences in the action of parent BDE-47 and its hydroxylated metabolites, 5-OH-BDE-47 and 6-OH-BDE47, on steroid secretion by luteal cells. Article Snippet: 13 In this study we determined the effects of BDE-47 on the expression and activity of phase I 14 (CYP2B1/2) and phase II (SULT1A and COMT) enzymes, and assessed the actions of BDE15 47 and its metabolites on luteal steroidogenesis.. Luteal cells collected during early (ELP), 16 middle (MLP) and late (LLP) luteal phase were exposed to BDE-47 (0.5, 25, and 50 ng/ml) or 17 metabolites (2.5, 5 and 25 ng/ml).. BDE-47 decreased CYP2B1/2 activity and expression but 18 had no effect on SULT1A or COMT. Article Title: Hexachlorobenzene and pentachlorobenzene accumulation, metabolism and effect on steroid secretion and on CYP11A1 and CYP19 expression in cultured human placental tissue. Article Snippet: Hexachlorobenzene and pentachlorobenzene accumulation and the effect on CYP1A1, SULT1A, COMT and steroid secretion in term placental tissue were determined.. Explants of placental tissue were exposed to between 0.02 and 2 ng/ml HCBz or PeCBz for 6–72 h. Accumulation was measured by capillary gas chromatography and quadrupole mass spectrometry.. CYP1A1, SULT1A, COMT activity and progesterone secretion were analysed by EIA. Article Title: Activation of the enzymes of phase I (CYP2B1/2) and phase II (SULT1A and COMT) metabolism by 2,2',4,4'-tetrabromodiphenyl ether (BDE47) in the pig ovary. Article Snippet: The aim of the current study was to determine metabolism of polybrominated diphenyl ether (BDE-47) in the porcine ovary.. We analyzed the activity and expression of enzymes involved in phase I (CYP1A1 and CYP2B1/2) and phase II (SULT1A and COMT) of BDE-47 metabolism.. Basal CYP1A1 and CYP2B1/2 activity increased during culture. Article Title: Nitrophenols disrupt the expression and activity of biotransformation enzymes (CYP3A and COMT) in chicken ovarian follicles in vivo and in vitro. Article Snippet: The reaction was terminated by the addition of 1 M NaOH, and absorbance was measured at a wavelength of 520 nm using a Article Title: A mixture of persistent organic pollutants detected in human follicular fluid increases progesterone secretion and mitochondrial activity in human granulosa HGrC1 cells. Article Snippet: Absorbance was measured at 490 nm using a Article Title: Nitrophenols disrupt the expression and activity of biotransformation enzymes (CYP3A and COMT) in chicken ovarian follicles in vivo and in vitro. Article Snippet: The absorbance was measured after a 30-min incubation period at a wavelength of 520 nm using a |
